KMID : 0525719990040020090
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Journal of Chitin and Chitosan 1999 Volume.4 No. 2 p.90 ~ p.95
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Hydrolytic Depolymerization of Chitosan using Organic Acids to Oligo - chitosaccharides
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ÃÖ±¤Áø/Choi GJ
±èÇö»ó/½É»óÁØ/±è¿µ´ë/¿ì°æÀÚ/Á¶¿µ»ó/Kim HS/Sim SJ/Kim YD/Woo KJ/Cho YS
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Abstract
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Chitosan has been degraded by chemical and enzymatic processes, and the efficiency and oligosaccharide distribution of the products were compared. When chemically hydrolyzed with 12 N HCI at 80 ¡É, the reaction product contained the monomer to octamer, in which the yield of high degree of polymerization (dp) chitooligosaccharides was low and glucosamine was identified as major product. When enzymatically hydrolyzed with Bacillus sp. P16 chitosanase, the reaction products contained dimer to nanomer, in which the yield of high dp chitooligosaccharides was relatively high, and Pentamer and hexamer were identified as major products. Thus, enzymatic processes have advantages over chemical hydrolysis in terms of the efficiency and distribution of high dp chitooligosaccharides.bacillus sp. P16 chitosanase was evaluated as a potential source for the production of high dp chitooligosaccharides from chitosan.
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KEYWORD
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